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教授

 

秦慧民,博士,yh1187银河网站教授。中国微生物学会会员。
学习经历:
2010.4-2013.3 东京大学应用生命化学专业 博士
2004.9-2008.7 华南理工大学制糖工程专业 硕士
2000.9-2004.7 山东农业大学食品科学与工程专业 本科
工作经历:
2017/12-至今 天津科技大学, 教授; 主要研究方向: 发酵工程;酶工程
2015/6-2017/11 天津科技大学, 讲师; 主要研究方向: 发酵工程;酶工程
2013/4-2015/5 东京大学应用生命化学专业 特任助教 
2008/10-2009/8 东北师范大学中国赴日本国留学生预备学校 日语学习
主讲课程:
本科生:微生物学实验,微生物遗传学实验
科研领域及方向
[1] 甾体药物微生物转化中相关酶系的结构与功能研究;
[2] 海藻酸多糖降解酶的结构与功能解析;
[3] 手性化合物的产业应用及其相关酶的结构解析,反应机理与功能改造研究;
[4] 人乳寡糖糖基转移酶的结构生物学研究。
科研情况
[1] 国家自然科学基金面上项目,维生素D3前体生物合成关键酶胆固醇7-脱氢酶系的构效研究,2018.1-2021.12,在研,主持项目
[2] 天津市自然科学基金青年项目,海藻酸多糖降解酶的结构生物学与功能研究,2016.4-2019.3, 在研,主持项目
[3] 天津市引进人才科研启动项目,工业化应用相关酶的结构生物学基础研究,2015.9-2017.12, 结题,主持项目
[4] 食品添加剂与配料绿色制造关键技术研究与开发,国家重点研发计划专项项目,2016-2020,在研,参与项目
[5] 益生菌健康功能与基于肠道微生物组学的食品营养代谢机理研究,国家重点研发计划专项,2017/07-2020/12,在研,参与项目
[6] 皮革关键酶制剂与生物技术及应用示范,国家重点研发计划专项,2017/07-2021/6,在研,参与项目
主要学术成果
[1] Qin, H.-M., Miyakawa, T., Inoue, A., Nishiyama, R., Nakamura, A., Asanoa, A., Ojima, T., Tanokura, M. Structural basis for controlling the enzymatic properties of polymannuronate preferred alginate lyase FlAlyA from the PL-7 family. Chem. Commun. 2018, 54: 555-558
[2] Qin, H.-M., Xu, P., Guo, Q., Cheng, X., Gao, D., Sun, D., Zhu, Z., Lu, F., Biochemical characterization of a novel ulvan lyase from Pseudoalteromonas sp. strain PLSV. RSC Adv. 2018, 8: 2610-2615
[3] Qin, H.-M., Miyakawa, T., Inoue, A., Nishiyama, R., Nakamura, A., Asano, A., Sawano, Y. Ojima, T., Tanokura, M. Structure and polymannuronate specificity in a eukaryotic member of the polysaccharide lyase family 14. J. Biol. Chem. 2017, 292, 2182-2190 
[4] Qi, Z., Zhu, Z., Wang, J.‑W., Li, S., Guo, Q., Xu, P., Lu,F. Qin, H.‑M. Biochemical analysis and the preliminary crystallographic characterization of D-tagatose 3-epimerase from Rhodobacter sphaeroides. Microb. Cell Fact. 2017,16: 193
[5] Qin, H.-M., Zhu, Z., Ma, Z., Xu, P., Guo, Q., Li, S., Wang, J.-W., Mao, S., Liu, F., Lu F. Rational design of cholesterol oxidase for efficient bioresolution of cholestane skeleton substrates. Sci. Rep. 2017, 7: 16375
[6] Qin, H.-M., Miyakawa, T., Inoue, A., Nakamura, A., Nishiyama, R., Ojima, T., Tanokura, M. Laminarinase from Flavobacterium sp. reveals the structural basis of thermostability and substrate specificity. Sci. Rep. 2017, 7, 11425
[7] Li, Y., Wei, L., Zhu, Z., Li, S., Wang, J.-W., Xin, Q., Wang, H., Lu F., Qin, H.-M. Rational design to change product specificities and thermostability of cyclodextrin glycosyltransferase from Paenibacillus sp. RSC Adv. 2017, 7, 13726-13732 
[8] Qin, H.-M., Wang, J.-W., Guo, Q., Li, S., Xu, P., Zhu, Z., Lu F. Refolding of a novel cholesterol oxidase from Pimelobacter simplex reveals dehydrogenation activity. Protein Expres. Purif. 2017, 139, 1-7
[9] Qin, H.-M., Li, S., Zhang, Y.-F., Wang, J.-W., Li, J., Song, S., Lu, F., Li, Y. Multienzymatic cascade synthesis of fucosyloligosaccharides via a two-step fermentation strategy in Escherichia coli. Biotechnol. Lett. 2016, 38, 1747-1752 
[10] Qin, H.-M., Imai, F. L., Miyakawa, T., Kataoka, M., Okai, M., Ohtsuka, J., Hou, F., Nagata, K., Shimizu, S. and Tanokura, M. L-allo-Threonine aldolase with H128Y/S292R mutation from Aeromonas jandaei DK-39 reveals the structural basis of change for substrate stereoselectivity. Acta Crystallogr. D, Biological Crystallography. 2014, 70, 1695-1703 
[11] Qin, H.-M., Yamamura, A., Miyakawa, T., Kataoka, M., Nagai, T., Kitamura, N., Urano, N., Maruoka, S., Ohtsuka, J., Nagata, K., Shimizu, S. and Tanokura, M. Structure of conjugated polyketone reductase from Candida parapsilosis IFO 0708 reveals conformational changes for substrate recognition upon NADPH binding. Appl. Microbiol. Biot. 2014, 98, 243-249
[12] Qin, H.-M., Miyakawa, T., Nakamura, A., Hibi, M., Ogawa, J., and Tanokura, M. Structural optimization of SadA, an Fe(II)- and a-ketoglutarate-dependent dioxygenase targeting biocatalytic synthesis of N-succinyl-L-threo-3,4-dimethoxyphenylserine. Biochem. Bioph. Res. Co. 2014, 450, 1458-1461 
[13] Nakamura, H.,* Xue, Y.-L.,* Miyakawa, T.,* Hou, F., Qin, H.-M., Fukui, K., Shi, X., Ito, E., Ito, S., Park, S.-H. Miyauchi, Y., Asano, A., Ueda, T., Tanokura, M., Asami. T. Molecular mechanism of strigolactone perception by DWARF14 in plant. Nature commun. 2013, 4, 2613
[14] Qin, H.-M., Miyakawa, T., Jia, M.-Z., Nakamura, A., Ohtsuka, J., Xue, Y.-L., Kawashima, T., Kasahara, T., Hibi, M., Ogawa, J. and Tanokura, M. Crystal structure of a novel N-substituted L-amino acid dioxygenase from Burkholderia ambifaria AMMD. PLOS ONE 2013, e63996 
[15] Qin, H.-M., Yamamura, A., Miyakawa, T., Kataoka, M., Maruoka, S., Ohtsuka, J., Nagata, K., Shimizu, S. and Tanokura, M. Crystal structure of conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 complexed with NADPH. Proteins 2013, 81,2059-2063 
[16] Qin, H.-M., Miyakawa, T., Nakamura, A., Xue, Y.-L., Kawashima, T., Kasahara, T., Hibi, M., Ogawa, J. and Tanokura, M. Expression, purification, crystallization and preliminary X-ray analysis of a branched-chain amino acid dioxygenase from Burkholderia ambifaria AMMD. Acta Crystallogr. F, Structural Biology and Crystallization Communications 2012, 68, 1067-1069
获奖情况
2009-2013 日本政府(文部科学省)国费奖学金——大使馆推荐
2013年 东京大学农学生命科学研究科长受赏
联系方式
办公地点:天津经济技术开发区第十三大街29号yh1187银河网站
邮政编码:300457
办公电话:022-60602949
Email: huiminqin@tust.edu.cn

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