Associate Professors

Xuewu Guo,Ph.D, Associate professor, College of Biotechnology, Tianjin University of Science and Technology, Master Supervisor

Ph.D., 2010-2014, Applied Chemistry, Tianjin University
M.S., 2002-2006 Biochemistry and Molecular Biology, Institute of Microbiology, Chinese Academy of Sciences, , China 

B.S. 1998- 2002, Biological Science, Hunan Normal University, China.

Research field 
[1] Modern brewing technology and food safety; 
[2] Bio-based chemicals and synthetic biology;

[3] Yeast biology

Current Research Projects 
[1] National Natural Science Foundation of China: Research on synthesis mechanism of RNA in Saccharomyces cerevisiae with high RNA content, 31671834, 2017-2020
[2] National Natural Science Foundation of China: Breeding of Klebsiella pneumoniae with efficient 2,3-butanediol production from xylose by directed evolution of cellular transcription σ and its regulation mechanism, 21406168, 2015-2017
[3] China Postdoctoral Science Foundation Project: Role and Mechanism of Actinomycetes in Baijiu Production, 2017M611169, 2017-2019
[4] Tianjin Applied Basic and Frontier Technology Research Program: Breeding of the best genetically engineered strains of high-yield ester Saccharomyces cerevisiae, 12JCQNJC06500, 2012-2015

[5] National Science and Technology Support Project Sub-project: Demonstration of Food Safety Source Control and Early Warning Technology Research and Promotion Application---Yeast Safety Production and Quality Control, 2014-2017

1.Guo, XW*; Zhang, Y; Li, LL; Guo, J; Wu, DG; Chen, YF; Xiao, DG, Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics, Biotechnol Biofuels, 2018,11:307 doi: 10.1186/s13068-018-1312-8
2.Li P, Gao Y, Wang C, Zhang CY, Guo X, Xiao D. Effect of ILV6 Deletion and Expression of aldB from Lactobacillus plantarum in Saccharomyces uvarum on Diacetyl Production and Wine Flavor. J Agric Food Chem. 2018,66(32):8556-8565. doi: 10.1021/acs.jafc.8b02356.
3.Li F, Yin C, Sun L, Li Y, Guo X, Song H. Synthetic Klebsiella pneumoniae-Shewanella oneidensis Consortium Enables Glycerol-Fed High-Performance Microbial Fuel Cells. Biotechnol J. 2018;13(5):e1700491. doi: 10.1002/biot.201700491.
4.Guo J, Huang S, Chen Y, Guo X, Xiao D. Heterologous expression of Spathaspora passalidarum xylose reductase and xylitol dehydrogenase genes improved xylose fermentation ability of Aureobasidium pullulans. Microb Cell Fact. 2018;17(1):64. doi: 10.1186/s12934-018-0911-1.
5.Li W, Chen SJ, Wang JH, Zhang CY, Shi Y, Guo XW, Chen YF, Xiao DG. Genetic engineering to alter carbon flux for various higher alcohol productions by Saccharomyces cerevisiae for Chinese Baijiu fermentation. Appl Microbiol Biotechnol. 2018; 102(4):1783-1795. doi: 10.1007/s00253-017-8715-5.
6.Guo J, Huang S, Chen Y, Guo X, Xiao D. Discovering the role of the apolipoprotein gene and the genes in the putative pullulan biosynthesis pathway on the synthesis of pullulan, heavy oil and melanin in Aureobasidium pullulans.  World J Microbiol Biotechnol. 2018;34(1):11. doi: 10.1007/s11274-017-2398-z.
7.Guo, XW*; Wang, YZ; Guo, J; Wang, Q; Zhang, Y ; Chen, YF; Zhang, CY; Xiao, DG, Efficient production of 2,3-butanediol from cheese whey powder (CWP) solution by Klebsiella pneumoniae through integrating pulsed fed-batch fermentation with a two-stage pH control strategy, Fuel, 2017, 203: 469-477 DOI: 10.1016/j.fuel.2017.04.138
8.Li P, Guo X*, Shi T, Hu Z, Chen Y, Du L, Xiao D*. Reducing diacetyl production of wine by overexpressing BDH1 and BDH2 in Saccharomyces uvarum. J Ind Microbiol Biotechnol. 2017;44(11):1541-1550. doi: 10.1007/s10295-017-1976-2.
9.Shi TT, Li P, Chen SJ, Chen YF, Guo XW*, Xiao DG*. Reduced production of diacetyl by overexpressing BDH2 gene and ILV5 gene in yeast of the lager brewers with one ILV2 allelic gene deleted. J Ind Microbiol Biotechnol. 2017;44(3):397-405. doi: 10.1007/s10295-017-1903-6. 
10.Song L, Chen Y, Du Y, Wang X, Guo X, Dong J, Xiao D. Saccharomyces cerevisiae proteinase A excretion and wine making. World J Microbiol Biotechnol. 2017;33(11):210. doi: 10.1007/s11274-017-2361-z.
11.Guo J, Wang Y, Li B, Huang S, Chen Y, Guo X, Xiao D. Development of a one-step gene knock-out and knock-in method for metabolic engineering of Aureobasidium pullulans. J Biotechnol. 2017, 251:145-150. doi: 10.1016/j.jbiotec.2017.04.029.
12.Yu H, Guo J, Chen Y, Fu G, Li B, Guo X, Xiao D. Efficient utilization of hemicellulose and cellulose in alkali liquor-pretreated corncob for bioethanol production at high solid loading by Spathaspora passalidarum U1-58. Bioresour Technol. 2017;232:168-175. doi: 10.1016/j.biortech.2017.01.077.
13.Chen Y, Song L, Han Y, Liu M, Gong R, Luo W, Guo X, Xiao D. Decreased proteinase A excretion by strengthening its vacuolar sorting and weakening its constitutive secretion in Saccharomyces cerevisiae. J Ind Microbiol Biotechnol. 2017;44(1):149-159. doi: 10.1007/s10295-016-1868-x. 
14.Guo XW, Li YZ, Guo J, Wang Q, Huang SY, Chen YF, Du LP, Xiao DG. Reduced production of ethyl carbamate for wine fermentation by deleting CAR1 in Saccharomyces cerevisiae. J Ind Microbiol Biotechnol. 2016;43(5):671-9. doi: 10.1007/s10295-016-1737-7. 
15.Chen Y, Luo W, Gong R, Xue X, Guan X, Song L, Guo X, Xiao D.  Improved ethyl caproate production of Chinese liquor yeast by overexpressing fatty acid synthesis genes with OPI1 deletion. J Ind Microbiol Biotechnol. 2016;43(9):1261-70. doi: 10.1007/s10295-016-1795-x.
16.Guo J, Li J, Chen Y, Guo X, Xiao D. Improving Erythritol Production of Aureobasidium pullulans from Xylose by Mutagenesis and Medium Optimization. Appl Biochem Biotechnol. 2016;180(4):717-727.
17.Shi, TT; Guo, XW ; Li, P; Zhou, Z; Xiao, DG, Diacetyl content reduction in industrial brewer's yeast through ILV2 disruption and BDH1 expression, European Food Research and Technology, 2016, 242(6): 919-926 DOI: 10.1007/s00217-015-2598-4
18.Zhang, CY; Li, W; Wang, DS; Guo, XW; Ma, LJ; Xiao, DG, Production of 2,3-butanediol by Enterobacter cloacae from corncob-derived xylose, Turkish Journal of Biology, 2016, 40(4): 856-865 DOI: 10.3906/biy-1506-66 
19.Guo X, Guan X, Wang Y, Li L, Wu D, Chen Y, Pei H, Xiao D. Reduction of biogenic amines production by eliminating the PEP4 gene in Saccharomyces cerevisiae during fermentation of Chinese rice wine. Food Chem. 2015;178:208-11. doi: 10.1016/j.foodchem.2015.01.089.
20.Chen, YF; Huang, WL; Fu, GX; Guo, J; Liu, MM; Guo, XW; Xiao, DG, A genetic transformation protocol for the xylose-fermenting yeast Spathaspora passalidarum, Engineering in Life Sciences,2015, 15(5): 550-555 DOI: 10.1002/elsc.201400232
21.Dong J, Xu H, Zhao L, Chen Y, Zhang C, Guo X, Hou X, Chen D, Zhang C, Xiao D. Enhanced acetate ester production of Chinese liquor yeast by overexpressing ATF1 through precise and seamless insertion of PGK1 promoter. J Ind Microbiol Biotechnol. 2014;41(12):1823-8. doi: 10.1007/s10295-014-1522-4. 
22.Guo X, Cao C, Wang Y, Li C, Wu M, Chen Y, Zhang C, Pei H, Xiao D. Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain. Biotechnol Biofuels. 2014;7(1):44. doi: 10.1186/1754-6834-7-44
23.Guo XW, Zhang YH, Cao CH, Shen T, Wu MY, Chen YF, Zhang CY, Xiao DG. Enhanced production of 2,3-butanediol by overexpressing acetolactate synthase and acetoin reductase in Klebsiella pneumoniae. Biotechnol Appl Biochem. 2014;61(6):707-15. doi: 10.1002/bab.1217
24.Zhang CY, Peng XP, Li W, Guo XW, Xiao DG. Optimization of 2,3-butanediol production by Enterobacter cloacae in simultaneous saccharification and fermentation of corncob residue. Biotechnol Appl Biochem. 2014;61(5):501-9. doi: 10.1002/bab.1198.
25.Chen Y, Li F, Guo J, Liu G, Guo X, Xiao D. Enhanced ethyl caproate production of Chinese liquor yeast by overexpressing EHT1 with deleted FAA1. J Ind Microbiol Biotechnol. 2014;41(3):563-72. doi: 10.1007/s10295-013-1390-3.
26.Chen, YF; Guo, J ; Li,; Liu, MM; Zhang, XX; Guo, XW; Xiao, DG, Production of pullulan from xylose and hemicellulose hydrolysate by Aureobasidium pullulans AY82 with pH control and DL-dithiothreitol addition, Biotechnology and Bioprocess Engineering, 2014, 19(2): 282-288 DOI: 10.1007/s12257-013-0715-4
27.Chen, YF; Zhang, XX; Zhang, SJ; Qin, WJ; Guo, CH; Guo, XW; Xiao, DG,Enhanced enzymatic xylose/cellulose fractionation from alkaline liquor-pretreated corn cob by surfactant addition and separate fermentation to bioethanol, Turkish Journal of Biology, 2014,38(4): 478-484 DOI: 10.3906/biy-1310-16
28.Zou J, Guo X, Shen T, Dong J, Zhang C, Xiao D. Construction of lactose-consuming Saccharomyces cerevisiae for lactose fermentation into ethanol fuel. J Ind Microbiol Biotechnol. 2013;40(3-4):353-63. doi: 10.1007/s10295-012-1227-5.
29.Wu D, Guo X, Lu J, Sun X, Li F, Chen Y, Xiao D. A rapid and efficient one-step site-directed deletion, insertion, and substitution mutagenesis protocol.  Anal Biochem. 2013;434(2):254-8. doi: 10.1016/j.ab.2012.11.028. 
30.Dong BY, Chen YF, Zhao CC, Zhang SJ, Guo XW, Xiao DG. Simultaneous determination of furfural, acetic acid, and 5-hydroxymethylfurfural in corncob hydrolysates using liquid chromatography with ultraviolet detection. J AOAC Int. 2013;96(6):1239-44.
31.Zhang, JW; Zhang, CY; Dai, LH; Dong, J; Liu, YL; Guo, XW; Xiao, DG, Effects of overexpression of the alcohol acetyltransferase-encoding gene ATF1 and disruption of the esterase-encoding gene IAH1 on the flavour profiles of Chinese yellow rice wine, International Journal of Food Science & Technology, 2012, 47(12): 2590-2596 DOI: 10.1111/j.1365-2621.2012.03140.x
32.Guo, XW; Wang, RS ; Chen, YF; Xiao, DG, Intergeneric yeast fusants with efficient ethanol production from cheese whey powder solution: Construction of a Kluyveromyces marxianus and Saccharomyces cerevisiae AY-5 hybrid, Engineering in Life Sciences, 2012,12(6): 656-661 DOI: 10.1002/elsc.201100112
33.Lu, J; Dong, J; Wu, DG; Chen, YF; Guo, XW; Shi, Y; Sun, X; Xiao, DG, Construction of recombinant industrial brewer's yeast with lower diacetyl production and proteinase A activity, European Food Research and Technology, 2012, 235(5): 951-961 DOI: 10.1007/s00217-012-1821-9
34.Chen Y, Yang X, Zhang S, Wang X, Guo C, Guo X, Xiao D. Development of Saccharomyces cerevisiae producing higher levels of sulfur dioxide and glutathione to improve beer flavor stability. Appl Biochem Biotechnol. 2012;166(2):402-13. doi: 10.1007/s12010-011-9436-3.
35.Guo X, Zhou J, Xiao D. Improved ethanol production by mixed immobilized cells of Kluyveromyces marxianus and Saccharomyces cerevisiae from cheese whey powder solution fermentation. Appl Biochem Biotechnol. 2010;160(2):532-8. doi: 10.1007/s12010-008-8412-z.
36.Xiao D, Wu S, Zhu X, Chen Y, Guo X. Effects of soya fatty acids on cassava ethanol fermentation. Appl Biochem Biotechnol. 2010;160(2):410-20. doi: 10.1007/s12010-008-8344-7.

College of Biotechnology, Tianjin University of Science and Technology, No. 29, St. No. 13 Tianjin Econmic-Technological Development Area (TEDA), Tianjin 300457, P. R. China 
Tel : +86 22 60601389
Fax: +86 22 60602298
E-mail: guoxuewu@tust.edu.cn